Description
Description: This assay employs a two-site sandwich ELISA to quantitate CER1 in samples. An antibody specific for CER1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CER1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CER1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CER1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: Cerberus also known as CER1 is a cytokine member of the cystine knot superfamily, characterized by nine conserved cysteines and a cysteine knot region. The cerberus-related cytokines, together with Dan and DRM / Gremlin, represent a group of bone morphogenetic protein (BMP) antagonists that can bind directly to BMPs and inhibit their activity.Cerberus is an inhibitor in the TGF beta signaling pathway secreted during the gastrulation phase of the embryogenesis.Cer1 mimicked the anti-BMP activity of Xcer in Xenopus animal caps, although with reduced potency. In situ hybridization to early gastrula mouse embryos revealed that Cer1 expression was restricted to the anterior primitive endoderm, the tissue thought to initiate head induction